- 作者: Jun Bao; Britto Nathan; Che-Chang Hsu; Yuan Zhang; Rosemary Wu; Jang-Yen Wu
- 作者服務機構: Department of Physiology and Cell Biology, University of Kansas, Lawrence, Kans., USA
- 中文摘要: --
- 英文摘要: In the brain, the γ-aminobutyric acid (GABA) level is primarily controlled by the activity of its synthesizing enzyme, L-glutamate decarboxylase (GAD). At present, mechanisms responsible for regulation of GAD activity remain large- 1y unknown. Here we report that GAD activity is inhibited by conditions favoring protein phosphorylation, and this inhibition can be reversed by phos- phatase treatment. Furthermore, this inhibition appears to result from the suppression of a Ca2+-dependent phosphatase. Phosphorylation of GAD is demonstrated by direct incorporation of 32P into the GAD protein. These results suggest that GAD activity in the brain is inhibited by phosphorylation and activated by dephosphorylation. A model for regulation of GABA synthe- sis related to neuronal excitation is discussed.
- 中文關鍵字: --
- 英文關鍵字: γ-Aminobutyric acid; L-glutamate decarboxylase; Protein phosphorylation; Neurotransmitter synthesis