- 作者: Chee-Hing Yang; Hui-Chun Li; Jeng-Geng Jiang; Che-Fang Hsu; Yi-Jen Wang; Meng-Jiun Lai; Yue-Li Juang; Shih-Yen Lo
- 作者服務機構: Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Enterovirus type 71 (EV71) 2A protease exhibited strong transcriptional activity
in yeast cells. The transcriptional activity of 2A protease was independent of its
protease activity. EV71 2A protease retained its transcriptional activity after
truncation of 40 amino acids at the N-terminus but lost this activity after truncation of
60 amino acids at the N-terminus or deletion of 20 amino acids at the C-terminus.
Thus, the acidic domain at the C-terminus of this protein is essential for its
transcriptional activity. Indeed, deletion of amino acids from 146 to 149 (EAME) in
this acidic domain lost the transcriptional activity of EV71 2A protein though still
retained its protease activity. EV71 2A protease was detected both in the cytoplasm
and nucleus using confocal microscopy analysis. Coxsackie virus B3 2A protease also
exhibited transcriptional activity in yeast cells. As expected, an acidic domain in the
C-terminus of Coxsackie virus B3 2A protease was also identified. Truncation of this
acidic domain resulted in the loss of transcriptional activity. Interestingly, this acidic
region of poliovirus 2A protease is critical for viral RNA replication. The
transcriptional activity of the EV71 or Coxsackie virus B3 2A protease should play a
role in viral replication and/or pathogenesis. - 中文關鍵字: --
- 英文關鍵字: --