- 作者: Mei-Ying Liu; Yuh-Lin Wu; Li-Sheng Chen; Tsuey-Ying Hsu; Jen-Yang Chen; Czau-Siung Yang
- 作者服務機構: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要: A 1.7-kb cDNA clone, pGEM-cDP, was isolated from a cDNA library of IUdR-induced p3HR1 cells. It contains the upstream nucleotide sequence of the Epstein-Barr virus (EBV) DNA polymerase gene from 156,859 to 155,088, and was subcloned into expression vector pET3cp* by the polymerase chain reaction, giving the plasmid pDP 1 .Using a T7 RNA polymerase expression system, a 77-kD polypeptide was produced from pDP 1 in Escherichia coli and specific hyperimmune serum was generated in mice. The truncated EBV DNA polymerase was shown to possess the authentic antigenicity by an indirect immunofluorescence assay and by immunoblotting using EBV-containing cells as antigens. Serum from nasopharyngeal carcinoma (NPC) patients and healthy donors was examined for antibodies against the 77-kD polypeptide by Western blot analyses and ELISAs. About 70% NPC patients were positive, while less than 15 % of healthy persons showed weak reactivities in ELISAs.
- 中文關鍵字: --
- 英文關鍵字: Epstein-Barr virus; DNA polymerase; Nasopharyngeal carcinoma