- 作者: He-Yen Chou, Yu-Hung Lin, Guan-Lin Shiu, Hsiang-Yu Tang, Mei-Ling Cheng, Ming-Shi Shiao, Li-Mei Pai
- 作者服務機構: Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan R.O.C
- 中文摘要: --
- 英文摘要:
Background:
Methionine, an essential amino acid, is required for protein synthesis and normal cell metabolism. The transmethylation pathway and methionine salvage pathway (MTA cycle)are two major pathways regulating methionine metabolism. Recently, methionine has been reported to play a key role in Drosophila fecundity.
Results:
Here, we revealed that the MTA cycle plays a crucial role in Drosophila fecundity using the mutant of aci-reductone dioxygenase 1 (DADI1), an enzyme in the MTA cycle. In dietary restriction condition, the egg production of adi1 mutant flies was reduced compared to that of control flies. This fecundity defect in mutant flies was rescued by reintroduction of Dadi1
gene. Moreover, a functional homolog of human ADI1 also recovered the reproduction defect, in which the enzymatic activity of human ADI1 is required for normal fecundity.Importantly, methionine supply rescued the fecundity defect in Dadi1 mutant flies. The detailed analysis of Dadi1 mutant ovaries revealed a dramatic change in the levels of methionine metabolism. In addition, we found that three compounds namely, methionine,SAM and Methionine sulfoxide, respectively, may be required for normal fecundity.
Conclusions:
In summary, these results suggest that ADI1, an MTA cycle enzyme, affects fly fecundity through the regulation of methionine metabolism. - 中文關鍵字: --
- 英文關鍵字: ADI1, Methionine, MTA cycle, Fecundity, Drosophila