- 作者: Ali Yazdanyar Michael Essmann Bryan Larsen
- 作者服務機構: Des Moines University Osteopathic Medical Center, Des Moines, Iowa, USA
- 中文摘要: --
- 英文摘要: Microbial virulence is generally considered to be multi-factorial with infection resulting from the sum of severalglobally regulated virulence factors. Estrogen may serveas a signal for global virulence induction in Candida albi-cans. Nonsteroidal estrogens and estrogen receptor an-tagonists may therefore have interesting effects on yeastand their virulence factors. Growth of C. albicans wasmonitored by viable plate counts at timed intervals afterinoculation into yeast nitrogen broth plus glucose. Todetermine if increased growth of yeast in the presence ofestradiol was due to tyrosine kinase-mediated signaling,we measured growth in the presence of genistein, estra-diol or genistein plus estradiol and compared these con-ditions to controls, which were not supplemented witheither compound. Unexpectedly, genistein stimulatedgrowth of C. albicans. In addition, genistein was found toincrease the rate of germination (possibly reflecting re-lease from Go into Gi cell cycle phase) and also increasedHsp90 expression, demonstrated by a dot blot techniquewhich employed a commercial primary antibody de-tected with chemiluminescence with horseradish peroxi-dase-labeled secondary antibody. These biological ef-fects may be attributable to genistein's activity as a phy-toestrogen. In contrast, nafoxidine suppressed growth ofCandida and mildly diminished Hsp90 expression. Thisstudy raises the possibility of receptor cross-talk be-tween estrogen and isoflavinoid compounds, and anties-trogens which may affect the same signaling system, though separate targets for each compound were notruled out.
- 中文關鍵字: --
- 英文關鍵字: Candida. Virulence. Estradiol. Enistein. Nafoxidine