- 作者: 眭台生 ; 林嘉能 ; 郭耿毓
- 作者服務機構: 中山大學化學系
- 中文摘要: Capillary zone electrophoresis with end-column amperometric detection at a copper microdisk electrode(100.mu.m in diameter) was successfully applied for simultaneous determination of carbohydrates and amino acids. Under the separation voltage of 27kV and the separation electrolyte of 80mM NaOH in a 75cm fused silica capillary(10.mu.m i.d.*375.mu.m o.d.), complete separation of a standard mixture containing 9 carbohydrates and 12 amino acids was achieved in less than 25 min. With the electrokinetical injection of 5.4s at 27kV and the detection potential of 0.62V vs. Ag/AgCl, the detection limits (S/N=3) were 0.22ppm-0.65ppm (1.2-1.9.mu.M) for carbohydrates and 0.31ppm-6.5ppm (2.3.mu.M-39.mu.M) for amino acids, respectively. The calculated numbers of theoretical plates were between 41,000 and 190,000. The use of this method for analysis of carbohydrates and amino acids in a urine sample was demonstrated.
- 英文摘要: --
- 中文關鍵字: Capillary Zone Electrophoresis; Copper Electrode; Carbohydrate; Amino Acid; Constant Potential Amperometric Detection
- 英文關鍵字: 毛細管帶狀電泳;銅電極;碳水化合物;胺基酸;定電壓電流滴定偵測