- 作者: 王來新 ; 楊定成 ; 呂有勇 等
- 作者服務機構: 北京醫科大學藥學院
- 中文摘要: Four oligonucleotides (15mer) and their conjugates in which psoralen was covalently linked to 5' end were synthesized. Compounds 16 and 20 contain the sequence which is complementary to the sense strand covering the first four codons and the upstream sequence close to the ribosome binding site of c-Ha-ras mRNA. It was found that compounds 16 and 20 inhibited the growth of cells that had been transformed by the c-Ha-ras plasmid activated c-Ha-ras oncogene. Compound 20 that carried psoralen was more efficient in inhibiting the growth of transformed cells than compound 16 without psoralen, suggesting that the psoralen might have increased permeability of oligodeoxynucleotides. A model experiment of duplex formation was pertinent to the idea that the inhibitory effect was caused by RNase H activity.
- 英文摘要: --
- 中文關鍵字: Oligodeoxynucleotide Conjugate; Anticancer; Oligonucleotide; Synthesis
- 英文關鍵字: 寡去氧核酸共軛物;抗癌;寡核酸;合成