- 作者: Rong-Yaun Shyu1,2, Chang-Chieh Wu3, Chun-Hua Wang4, Tzung-Chieh Tsai5, Lu-Kai Wang6, Mao-Liang Chen7, Shun-Yuan Jiang7* and Fu-Ming Tsai7*
- 作者服務機構: Department of Internal Medicine, Buddhist Tzu Chi General Hospital Taipei Branch, New Taipei City, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要:
Background: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined.
Results: In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion.
Conclusions: These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes. - 中文關鍵字: --
- 英文關鍵字: Murine H-rev107, Human H-REV107, Retinoid-inducible gene 1, Prostaglandin D2 synthase, Testis, HREV107 type II tumor suppressor