- 作者: Ann-Ping Tsou Yu-Chi Chuang Jin-Yuan Su Chu-Wen Yang Yu-Lun Liao Wei-Kuang Liu Jen-Hwey Chiu Chen-Kung Chou
- 作者服務機構: Institute of Biotechnology in Medicine, Department of Life Science, Institute of Biochemistry, Institute of Microbiology and Immunology, Institute of Traditional Medicine, National Yang-Ming University; Department of Medical Reserch and Education, Veterans General Hospital; Division of Molecular and Genomic Medicine, National Health Research Institutes, Nankang, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: Many of the promising applications of the microarraytechnology are pertinent to identifying abnormalities ingene expression that contribute to malignant progres-sion.We developed a bioinformatics tool to identify dif-ferentially expressed genes in human hepatocellular car-cinoma(HCC).This involved the construction of a liverEST database(http://lestdb.nhri.org.tw) and in silico ver-ification of differentially expressed genes with a humanhepatoma microarray database. The stringency of thesearch was reinforced with a statistical analysis. A novelimprinted gene, Paternally Expressed 10 (PEG10) wasidentified as having an elevated level of expression in themajority of the HCC samples and was also induced toexpress during G2/M phase of regenerating mouse liver.Ectopic expression of PEG10 in 293T cells affects cellcycle progression.PEG10 is distributed in the cytosoland associates with the nuclear membrane.This is thefirst time that an imprinted gene has been found to reex-press in both human HCC and in the regenerating mouseliver. This result indicates that the induction of the pater-nally imprinted gene may play an important role duringliver regeneration or carcinogenesis of the human hepa-tocyte.Understanding the molecular basis of the abnor-mal imprinting of PEG10 will shed new light on the pro-cess that leads to liver disease.
- 中文關鍵字: --
- 英文關鍵字: Imprinted gene. PEG10. HCC. Liver regeneration. Cell cycle regulator. Microarrays. Bioinformatics