- 作者: Elizabeth A. Rich, Jan M. Orenstein, Kuan-Teh Jeang
- 作者服務機構: Department of Medicine , Case Western Reserve Universitv , Cleveland , Ohio , Department of Pathology George Washington University , Washington , D. C. ,Molecular Virology Section, Laboratory of Molecular Microbiology , Nationa Institute of Allergy and Infectious Diseases , National Institutes of Heafth , Bethesda , Md., USA
- 中文摘要: --
- 英文摘要: We describe the construction of a macrophage-tropicHIV-1 molecular clone, pNLAD8-EGFP, which expressesenhanced green fluorescent protein. We show thatNLAD8-EGFP can infect monocyte-derived macrophagesas well as alveolar macrophages.NLAD8-EGFP-infectedmacrophages can be easily and sensitively detectedbased on the visualization of intracellular green fluores-cent protein.
- 中文關鍵字: --
- 英文關鍵字: HIV-1, Alveolar macrophages, Blood monocytes, Enhanced green fluorescent protein, AIDS, Cigarette smoking