- 作者: christy M. Sapp Ting Li Jiayou Zhang
- 作者服務機構: Deparment of Microblology and Immunology, Markey Cancer Center, University of Kentucky, Lexington, Ky., USA
- 中文摘要: --
- 英文摘要: Retroviral vectors usually contain drug resistance genes,which are used to select for infected cells and to deter-mine the viral titers. The viral tite「is referred to as colo-ny-forming units (CFUs). Color reporter genes, such asthe 1acZ gene and the green fluorescent protein gene(gfp), have been widely used as markers in retroviral vec-tors. In this report, a simple and rapid method for thedetermination of retroviral titers has been developed.The number of viral particles capable of forming individ-ual green cells per unit volume is defined as marker-forming units (MFUs) .The MFUs determined by usinggfp as a marker were found to be proportional to theCFUs obtained by using drug selection for five differentdrug resistance genes. In addition, after adjusting thetime factor, the MFUs are higher than CFUs in virusesreleased from 30 stable helper cell lines. The lower titersdetermined by CFUs are likely due to the toxicity ontransduced cells.
- 中文關鍵字: --
- 英文關鍵字: Retrovirus. Vector. Color reporter gene. Green Fluorescent protein. titer. Colony-forming unit. Marker-forming unit