• 作者： 陳英俊
• 作者服務機構： 高雄醫學院藥理學科
• 中文摘要： Mugiline β取自烏魚精巢'依Kossel之改良方法抽取而獲得其粗硫酸鹽之後，再經Dowex l-x8，CM-Cellulose 及CM-Sephadex C-25之柱層分離得以純化。以Sephadex G-75膠過濾實驗得知其分子量約略6200 (單體）及12400 (雙體）又以SDS-polyacrylamide gel electrophoresis得知約?6000 (單體）及12000 (雙體），除了含有大量的A-rginine之外仍含有微量的Methionine, aspartic acid和lysine?其特異之處，由電泳，膠過濾及光學分析等方法可 知其物理化學性質與其他魚精蛋白類似。除了氨基酸分析及中和滴定曲線測定法之外，?無良好的鑑別方法用以分別不同 魚類之魚精蛋白。 25μg /ml之烏魚魚精蛋白即可使蛙心及離體天笠鼠之心房收縮。累積高劑量之投與亦使之發生收縮後之停止。5mg /kg可使家兔之呼吸頻率增快並降低其血壓。0.125 μg/ml可延長試管內家兔全血之血液凝固時間，並中和肝素之 抗凝血作用，62.5μg/ml以上即可在試管內抑制Collagen (膠原蛋白）引起血小板凝集。烏魚之魚精蛋白如同其他魚 類之魚精蛋白有希望成?藥典記載上，魚精蛋白關連製劑諸如Protamine-insulin及heparin antidote之原料。
• 英文摘要： Mugiline β is the basic polypeptide purified from the sperm nuclei of the bora testis. The molecular weight of purified mugiline β estimated by gel filtration through Sephadex G-75 is 6200 for the monomer but is 12,400 for the dimer, and that estimated by SDS-polyacrylamide gel electrophoresis is 6000 and 12,000 respectively. Traces of methionine, aspartic acid and lysine have been found in this arginine-rich protein and are specifically different from other protamines. Electrophoresis patterns and spectroanalysis of mugiline β indicate that physical properties of mugiline β are similar to those of other protamines. There is no other method to distinguish mugiline β from other protamines except by amino acid analysis and titration-behavior determination. Mugiline β at 25 μg/ml produces an augmentation in the contratility of isolated frog's heart and atrium of guinea pig. Systolic arrest was also observed in accumulative high doses. In the rabbit it produces remarkable changes in blood pressure and respiration. Above 5 mg/kg, it increases the respiratory frequency but decreases the respiratory amplitude and also lowers the blood pressure. Above 0.125 mg/ml, it prolongs the clotting time of rabbit blood in vitro and induces heparin neutralizing activity. Above 62.5 μg/ml, it also inhibits collagen-induced platelet aggregation in vitro.
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