- 作者: Shujun Li; Zhigang Li; Fengjie Guo; Xuebo Qin; Bin Liu; Zhe Lei; Zuoqing Song; Liya Sun; Hongtao Zhang; Jiacong You; Qinghua Zhou
- 作者服務機構: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China
- 中文摘要: --
- 英文摘要:
Background:Artemin (ARTN) is a neurotrophic factor belonging to the glial cell-derived
neurotrophic factor family of ligands. To develop potential therapy targeting ARTN, we studied
the roles of miR-223 in the migration and invasion of human esophageal carcinoma.
Methods: ARTN expression levels were detected in esophageal carcinoma cell lines KYSE-150,
KYSE-510, EC-9706, TE13,esophageal cancer tissues and paired non-cancerous tissues by
Western blot. Artemin siRNA expression vectors were constructed to knockdown of artemin
expression mitigated migration and invasiveness in KYSE150 cells. Monolayer wound healing
assay and Transwell invasion assay were applied to observe cancer cell migration and invasion.
The relative levels of expression were quantified by real-time quantitative PCR .
Results:
ARTN expression levels were higher in esophageal carcinoma tissue than in the adjacent tissue
and was differentially expressed in various esophageal carcinoma cell lines. ARTN mRNA
contains a binding site for miR-223 in the 3’UTR. Co-transfection of a mir-223 expression vector
with pMIR-ARTN led to the reduced activity of luciferase in a dual-luciferase reporter gene assay,
suggesting that ARTN is a target gene of miR-223. Overexpression of miR-223 decreased
expression of ARTN in KYSE150 cells while silencing miR-223 increased expression of ARTN
in EC9706 cells. Furthermore, overexpression of miR-223 in KYSE150 cells decreased cell
migration and invasion. Silencing of miR-223 in EC9706 cells increased cell migration and
invasiveness.
Conclusions: These results reveal that ARTN, a known tumor metastasis-related gene, is a direct
target of miR-223 and that miR-223 may have a tumor suppressor function in esophageal
carcinoma and could be used in anticancer therapies. - 中文關鍵字: --
- 英文關鍵字: miR-223;ARTN;Esophageal carcinoma;Migration and invasion