- 作者: Daejin Kim; Talia Hoory; Archana Monie; Annie Wu; Wei-Ting Hsueh; Sara I Pai; Chien-Fu Hung
- 作者服務機構: Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
- 中文摘要: --
- 英文摘要:
Background: Ovarian cancer is the leading cause of death among women with gynecologic
malignancies in the United States. Advanced ovarian cancers are difficult to cure with the current
available chemotherapy, which has many associated systemic side effects. Doxorubicin is one
such chemotherapeutic agent that can cause cardiotoxicity. Novel methods of delivering
chemotherapy without significant side effects are therefore of critical need.
Methods: In the current study, we generated an irradiated tumor cell-based drug delivery system
which uses irradiated tumor cells loaded with the chemotherapeutic drug, doxorubicin.
Results: We showed that incubation of murine ovarian cancer cells (MOSEC) with doxorubicin
led to the intracellular uptake of the drug (MOSEC-dox cells) and the eventual death of the
tumor cell. We then showed that doxorubicin loaded MOSEC-dox cells were able to deliver
doxorubicin to MOSEC cells in vivo. Further characterization of the doxorubicin transfer
revealed the involvement of cell contact. The irradiated form of the MOSEC-dox cells were
capable of treating luciferase-expressing MOSEC tumor cells (MOSEC/luc) in C57BL/6 mice as
well as in athymic nude mice resulting in improved survival compared to the non drug-loaded
irradiated MOSEC cells. Furthermore, we showed that irradiated MOSEC-dox cells was more
effective compared to an equivalent dose of doxorubicin in treating MOSEC/luc tumor-bearing
mice. - 中文關鍵字: --
- 英文關鍵字: --