- 作者: Sherin Antony; Peeyush T Kumar; Jobin Mathew; Anju T R; C S Paulose
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, Kerala, India
- 中文摘要: --
- 英文摘要:
Glucose homeostasis in humans is an important factor for the functioning of
nervous system. Hypoglycemia and hyperglycemia is found to be associated with central
and peripheral nerve system dysfunction. Changes in acetylcholine receptors have been
implicated in the pathophysiology of many major diseases of the central nervous system
(CNS). In the present study we showed the effects of insulin induced hypoglycemia and
streptozotocin induced diabetes on the cerebellar cholinergic receptors, GLUT3 and
muscle cholinergic activity. Results showed enhanced binding parameters and gene
expression of Muscarinic M1, M3 receptor subtypes in cerebellum of diabetic (D) and
hypoglycemic group (D + IIH and C + IIH). α7nAchR gene expression showed a
significant upregulation in diabetic group and showed further upregulated expression in
both D + IIH and C + IIH group. AchE expression significantly upregulated in
hypoglycemic and diabetic group. ChAT showed downregulation and GLUT3 expression
showed a significant upregulation in D + IIH and C + IIH and diabetic group. AchE
activity enhanced in the muscle of hypoglycemic and diabetic rats. Our studies
demonstrated a functional disturbance in the neuronal glucose transporter GLUT3 in the
cerebellum during insulin induced hypoglycemia in diabetic rats. Altered expression of
muscarinic M1, M3 and α7nAchR and increased muscle AchE activity in hypoglycemic
rats in cerebellum is suggested to cause cognitive and motor dysfunction. Hypoglycemia
induced changes in ChAT and AchE gene expression is suggested to cause impaired
acetycholine metabolism in the cerebellum. Cerebellar dysfunction is associated with
seizure generation, motor deficits and memory impairment. The results shows that cerebellar cholinergic neurotransmission is impaired during hyperglycemia and
hypoglycemia and the hypoglycemia is causing more prominent imbalance in cholinergic
neurotransmission which is suggested to be a cause of cerebellar dysfunction associated
with hypoglycemia. - 中文關鍵字: --
- 英文關鍵字: --