- 作者: Kang Fang; Tapas Mukhopadhyay; Stephen H. Shih
- 作者服務機構: a Department of Biology, National Taiwan; Normal University, Taipei, Taiwan, ROC;; b Department of Thoracic and; Cardiovascular Surgery, M.D. Anderson; Cancer Center, Houston, Tex., USA
- 中文摘要: --
- 英文摘要: In human lung epithelial cancer cell line H460, an accumulation of epidermal growth factor receptors (EGF-R) was observed following treatment with 1μM retinoic acid. An increase in 125I_labeled EGF binding capacity was detected, which reached its highest level after 48 h of incubation with retinoic acid. Transiently increased autophosphorylation of EGF-R after 48 h of retinoic acid treatment correlated with enhancement of EGF binding capacity on the H460 cell surface. Nuclear run-on analysis indicated that retinoic acid up- regulates transcription of the EGF-R gene, reaching a maximum at 48 h and decreasing after 72 h of treatment. When retinoic acid-treated cells were chased in drug-free medium, the increased EGF-R transcript level remained unchanged. Up-regulated expression of EGF-R in H460 cells is reflected by their increasing tumorigenicity phenotype. The results demonstrate that reti- noic acid induces EGF-R synthesis in human lung cancer cells.
- 中文關鍵字: --
- 英文關鍵字: Human lung cancer cells; Epidermal growth factor receptor; Retinoic acid