• 作者： 李龍湖
• 作者服務機構： 國立中興大學獸醫系
• 中文摘要： 以酵素接合免疫吸附法(ELISA)，間接螢光抗體法(IFA）及病毒中和試驗(V N)鑑定出9株具有傳染性華氏囊病毒4OKd蛋白衣殼反應之單源抗體(Mabs)，依病毒中和試驗結果可將該9株抗體分為兩群。第一群Mabs具中和病毒之能力，但第二群則否。利用這些Mabs做抗體－抗原之競爭結合試驗(Competitive binding assay），結果顯示40Kd蛋白衣殼至少含有2個抗體結合位(A及B)。第一群Mabs可和A結合，而另一群Mabs則和結合位B反應。將病毒蛋白衣殼變性處理後，第一群Mabs即失去和A位之結合反應，顯示和中和作用有關之結合位與蛋白質之三次元結構有密切關係(Conformation-dependent)。另於ELISA和病毒中和試驗結果顯示第一群Mabs與結合位A內之同一個epitope反應，但第二群Mabs則可與結合位B內之2或3個epitope反應。
• 英文摘要： Nine monoclonal antibodies (Mab) against a 40 Kd capsid protein of infectious bursal disease virus(IBDV) strain P3009 were isolated. They were characterized by enzyme-linked immunosorbent assay,indirect fluorescent antibody staining and virus neutralization. They were divided into two groups con-cerning virus neutralization. Group I Mabs were able to neutralize virus infectivity; however, group IIMabs were not. Competitive binding assays using these Mabs demonstrated the existence of two distinctantigenic regions (A and B) on the 40 Kd protein. Region A was recognized by group I Mabs and regionB was by group II Mabs. The binding reaction with group I Mabs was affected by denaturing of the viralproteins, indicating that the antigenic region involving neutralization was conformation-dependent. Theresults of enzyme-linked immunosorbent assays and virus neutralization tests suggested that group I Mabsmight react with one epitope within region A and group 11 Mabs with 2 or 3 epitopes within region B.
• 中文關鍵字： infectious bursal disease (IBD) virus; 40 Kd capsid protein monoclonal antibody; anti-body binding site
• 英文關鍵字： --