• 作者： 謝佳珍; 曾哲明
• 作者服務機構： 國立臺灣師範大學生物學系
• 中文摘要： 本研究之目的在證明腹腔巨噬細胞有調節B淋巴球生長與分泌抗體的功能。以酯多醣體活化後之老鼠脾臟B淋巴球培養在含有50%巨噬細胞（經酯多醣體刺激）培養基上清液後，發現B細胞之生長被巨噬細胞上清液抑制64%，IgM之分泌也被抑制50%，但是IgA之分泌反而增加2.6倍。當活化之B淋巴球培養在含轉型生長素-β1(TGF-β1)之培養基時，B細胞生長與IgM之分泌也受到抑制，但是IgA分泌增加了7.5倍，以螢光免疫法分析發現巨噬細胞上清液與TGF-β1顯著促進了表面IgA之表現。若將抗TGF-β1之抗體加入巨噬細胞上清液中，則巨噬細胞上清液既減少對B細胞生長與IgA分泌及表現的影響，以上證據證明巨噬細胞可經由分泌之TGF-β調節刀淋巴球之生長與抗體之分泌。
• 英文摘要： The regulatory effects of peritoneal macrophage culture supernatant on B-cell growth and IgA secretionwere demonstrated in this study. When the LPS-stimulated murine splenic B cells were cultured in mediumcontaining 50% LPS-activated macrophage culture supernatant, both the cell growth and IgM secretion ofB cells were suppressed by 64% and 50%, respectively, whereas the IgA secretion was increased 2.6-fold.Transforming growth factor-β1 (TGF-β1) is a potent regulator of IgA response. Macrophage culture super-natants contained on average 78 ng/ml of TGF-β1. When directly added to cultures, TGF-β1 also actedas a suppressive factor on B cell growth (55%) but induced an 7.5-fold increase in IgA secretion. Bothmacrophage culture supernatant and TGF-β1 induced an increase in the expression of surface IgA. Furtherstudy indicated that the augmenting effect of the macrophage culture supernatant on IgA secretion couldbe inhibited (50%) by an anti-TGFβ antibody, and that the suppressive effect of the macrophage culturesupernatant on the B-cell growth could be overcome by the same antibody, suggesting that TGF-β1 wasone of the cytokines in the macrophage culture supernatant that regulated B-cell function.
• 中文關鍵字： lgA Secretion; Macrophages; TGFβ1
• 英文關鍵字： --