• 作者： 曾成槐; 莊明維; 王聲遠; 謝瑞坤; 劉家瑞; 樊聖; 陳博明
• 作者服務機構： 臺北榮民總醫院內科部腫瘤科; 臺北榮民總醫院醫研部
• 中文摘要： 利用 14 位不同診斷之癌症病人及 2 位正常人週邊血液單核細胞，在不添加細胞分裂因子的情況，吾人成功地製備了淋巴激素活化殺手（LAK）細胞。以4小時51鉻－釋放測定法，上述兩類來源的 LAK 細胞都能表現出一定程度的細胞毒殺力。其作用的靶細胞包括：對自然殺手（NK）細胞具敏感的 K562 骨髓性白血病細胞株，抗自然殺手細胞的 Raji 淋巴瘤細胞株，及對化學治療已不具反應之病患的新鮮或冷凍白血病細胞等等，而對正常血球則無此毒性。此等細胞毒性，不管其反應的靶細胞為何，皆隨作用細胞與靶細胞比值之增高而加強，即100:1＞50:1＞25:1（P＜0.01）。 LAK 細胞與異體（9例）或自體（3例）骨髓細胞混合培養，以半固態瓊脂細胞群落形成（CFU-GM）測定法觀之，並不對已定向之造血幹細胞構成任何傷害。這些發現指出 LAK 細胞在自體骨髓移植前所進行的體外骨髓殘留白血病細胞清除過程上，或可扮演一相當積極之角色，值得進一步研究探討。
• 英文摘要： Lymphokine-activated killer (LAK) cells were generated successfully without mitogen from bloodmononuclear cells obtained from 14 patients with varying malignancies and 2 normal donors. Cells fromboth groups showed a positive cytotoxicity by a 4-hour Cr-release assay against a variety of target cellsincluding natural killer (NK) sensitive K562 myeloid leukemia, NK-resistant Raji lymphoma cell lines, andfresh/cryopreserved leukemia cells from patients refractory to standard chemotherapy but not normalblood cells. Higher cytotoxic activity was obtained with a higher effector:target ratio at 100:1＞50:1＞25:1 (P＜0.01) in each setting of different targets. Experiments involving cocultures of the LAK cells with either allogeneic (9) or autologous (3) bonemarrow cells disclosed no detrimental effect on the committed hemopoietic stem cells by semisolid agarcolony forming unit (CFU-GM) assay. The findings suggest that LAK cells may have a potential rolefor the in vitro purging of the residual leukemic cells from the marrow inoculum prepared for autologousbone marrow transplantation.
• 中文關鍵字： LAk cells; bone marrow transplantation; autologous
• 英文關鍵字： --