- 作者: Chung-Ming Chen, Yu-Chen S. H. Yang, Hsiu-Chu Chou & Shan Lin
- 作者服務機構: 1.Biotech Research Institute, Grape King Bio Ltd., Taoyuan, Taiwan 2.Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan 3.Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan 4.Department of Pediatrics, Taipei Medical University Hospital, Taipei, Taiwan 5.Joint Biobank, Office of Human Research, Taipei Medical University, Taipei, Taiwan 6.TMU Research Center for Digestive Medicine, Taipei Medical University, Taipei, Taiwan
- 中文摘要:
- 英文摘要:
Background
Supplemental oxygen impairs lung development in newborn infants with respiratory distress. Lactobacillus johnsonii supplementation attenuates respiratory viral infection in mice and exhibits anti-inflammatory effects. This study investigated the protective effects of intranasal administration of L. johnsonii on lung development in hyperoxia-exposed neonatal mice.
Methods
Neonatal C57BL/6N mice were reared in either room air (RA) or hyperoxia condition (85% O2). From postnatal days 0 to 6, they were administered intranasal 10 μL L. johnsonii at a dose of 1 × 105 colony-forming units. Control mice received an equal volume of normal saline (NS). We evaluated the following four study groups: RA + NS, RA + probiotic, O2 + NS, and O2 + probiotic. On postnatal day 7, lung and intestinal microbiota were sampled from the left lung and lower gastrointestinal tract, respectively. The right lung of each mouse was harvested for Western blot, cytokine, and histology analyses.
Results
The O2 + NS group exhibited significantly lower body weight and vascular density and significantly higher mean linear intercept (MLI) and lung cytokine levels compared with the RA + NS and RA + probiotic groups. At the genus level of the gut microbiota, the O2 + NS group exhibited significantly higher Staphylococcus and Enterobacter abundance and significantly lower Lactobacillus abundance compared with the RA + NS and RA + probiotic groups. Intranasal L. johnsonii treatment increased the vascular density, decreased the MLI and cytokine levels, and restored the gut microbiota in hyperoxia-exposed neonatal mice.
Conclusions
Intranasal administration of L. johnsonii protects against hyperoxia-induced lung injury and modulates the gut microbiota. - 中文關鍵字:
- 英文關鍵字: Probiotics, Hyperoxia, Mean linear intercept, Vascular endothelial growth factor, Microbiota