• 作者： 候連團; 劉謙美; 王敏瑩
• 作者服務機構： 國立臺灣大學醫學院牙醫學系暨牙醫科學研究所牙周病學組
• 中文摘要： 細胞外基質蛋白在牙周組織傷口痊癒過程中被認為是影響細胞附著、移動和增生分化的因子。本研究探討牙齦及牙周韌帶纖維細胞之基質蛋白合成能力，並觀察不同細胞培養密度對基質蛋白合成能力之影響。研究中使用免疫螢光檢測法測量上述二種細胞表現第一、第三型膠原蛋白和粘蛋白(fibronectin)的能力；部分細胞外基質蛋白亦經電泳和密度測量儀（densitometer)分析。實驗結果顯示牙齦和牙周韌帶纖維細胞分株都有製造第一、三型膠原蛋白和粘蛋白的能力，各分株細胞之細胞外基質蛋白之合成能力都不相同。牙周韌帶纖維分株細胞之基質蛋白合成能力大於牙齦纖維分株細胞。而且在低細胞密度培養時，每個細胞之蛋白合成能力要高於滿細胞密度培養狀態的。本研究證明牙周韌帶細胞在細胞內和基質蛋白的合成能力不同於牙齦細胞；兩種細胞之基質蛋白合成能力受到細胞培養密度的影響。
• 英文摘要： Extracellular matrix (ECM) proteins have been implicated in the attachment, migration and dif-ferentiation of cells during periodontal wound healing. This study was designed to investigate the expressionof ECM proteins in gingival fibroblasts (GF) and periodontal ligament fibroblasts (PF) at different cellgrowth densities. Both mass-cultured and cloned cells were derived from explants of healthy human gingivaeand periodontal ligament. The grown cells, in situ, were subjected to immunofluorescent staining for theexpression of collagen type I (CI), III (CIII) and fibronectin (Fn), and then measured by microfluorimetry.Partial biochemical measurement of the ECM proteins secreted in the medium was done by SDS-PAGEand densitometry. These data indicated that CI, CIII and Fn were expressed in both mass-cultured andcloned GF and PF. There were variations in the expression of these ECM proteins among the clones. PFand most of its clones produced a greater amount of total cellular and ECM proteins than did GF. Theexpression of these proteins was found to be greater in areas of low, as opposed to high, cell density.Parallel results were also noted in limited biochemical analyses. Thus, PF differed from GF with regardto the production of cellular and matrix proteins, and protein metabolism was affected by cell growthdensity. These data tend to support the previous hypothesis that PF is the essential cell type contributingto periodontal regeneration.
• 中文關鍵字： matrix proteins; cloned cells; periodontal ligament; gingival fibroblast; collagen; fibronectin (Fn); microfluorimetry.
• 英文關鍵字： --