- 作者: Chih-Pei Lin; Yung-Hsiang Chen; Jaw-Wen Chen; Hsin-Bang Leu; Tsan-Zon Liu; Po-Len Liu; Song-Lih Huang
- 作者服務機構: 1 Institute of Public Health, National Yang-Ming University, No. 155, Sec. 2, Linong St., Peitou, Taipei, 112, Taiwan; ; 2 Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; ; 3 Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; ; 4 Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan; ; 5 Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan; ; 6 Department and Institute of pharmacology, National Yang-Ming University, Taipei, Taiwan; ; 7 Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan; ; 8 Faculty of Respiratory Therapy, Kaohsiung Medical University, Kaohsiung, Taiwan
- 中文摘要: --
- 英文摘要: Hyperhomocysteinemia is associated with dysfunction and an independent risk factor of cardiovascular diseases. Cholestin (Monascus purpureus-fermented rice), contains a naturally-occurring statin, which has lipid-modulating and anti-inflammatory effects. We investigated the effects of Cholestin extract on the expression of vascular cell adhesion molecule-1 (VCAM-1) by homocysteine (HCY)-treated human aortic endothelial cells (HAECs). Supplement of HAECs with Cholestin extract significantly suppressed cellular binding between the human monocytic cells U937 and HCY-stimulated HAECs. Quantitative PCR and immunoblot analysis showed that Cholestin extract significantly attenuated HCY-induced expression of VCAM-1 mRNA and protein, respectively. Gel shift assays showed that Cholestin treatment reduced HCY-activated transcription factor nuclear factor-κΒ (NF-κΒ). Furthermore, Cholestin also attenuated reactive oxygen species (ROS) generation in vitro and in HCY-treated HAECs. Supplement with statins including simvastatin and parastatin gave similar results as compared with Cholestin. In conclusion, Cholestin reduces HCY-stimulated endothelial adhesiveness as well as downregulating intracellular ROS formation, NF-κΒ activation, and VCAM-1 expression in HAECs, supporting the notion that the natural compound Cholestin may have potential implications in clinical atherosclerosis disease.
- 中文關鍵字: --
- 英文關鍵字: Cell adhesion molecule, Cholestin (Monascus purpureus rice red yeast rice), Homocysteine, Nuclear factor-κΒ, Oxidative stress, Statins