- 作者: Li-Fang Wang; Jau-Shiuh Chen; Chih-Jung Hsu; Ching-Yi Liu; Jhang-Sian Yu; Shi-Chuen Miaw
- 作者服務機構: Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Exposure to protein allergen epicutaneously, inducing a Th2-dominant immune
response, sensitizes the host to the development of atopic disease. Antigen-driven
bystander effect demonstrates that polarized T cells could instruct naïve T cells to
differentiate into T cells with similar phenotype. In this study, we aimed to determine
the contribution of antigen-driven bystander effect on epicutaneous sensitization with
a newly introduced protein allergen. BALB/c mice were immunized intraperitoneally
with BSA emulsified in alum, known to induce a Th2 response, three weeks before
given BSA and OVA epicutaneously. Lymph node cells from these mice restimulated
with OVA secreted higher levels IL-4, IL-5 and IL-13 as compared with cells from
mice without BSA immunization. In addition, BALB/c mice immunized
subcutaneously with BSA emulsified in complete Freund’s adjuvant, known to induce
a Th1-predominant response, also induced higher Th1 as well as Th2 cytokine
response when restimulated with OVA as compared with mice without immunization.
We demonstrated that subcutaneous immunization with BSA in CFA induced Th2 as
well as Th1 response. The threshold of epicutaneous sensitization to OVA was also
reduced, possibly due to increased expressions of IL-4 and IL-10 in the draining
lymph nodes during the early phase of sensitization. In conclusion, antigen-driven
bystander effect, whether it is of Th1- or Th2-predominant nature, can accelerate epicutaneous sensitization by a newly introduced protein allergen. These results
provide a possible explanation for mono- to poly-sensitization spread commonly
observed in atopic children. - 中文關鍵字: --
- 英文關鍵字: --