• 作者： 朱俊
• 作者服務機構： 德州萊斯大學生化系
• 中文摘要： 如前次報告所示：85%之15-ketosterol靜脈注射針劑排於?汁中，令吾人臆測，肝臟為主要之器官，司其代謝，並
  將其代謝物排於?汁中。
  經由pulse-chase實驗，證明肝腫瘤細胞（Hep G2），確具此功能，它能充分吸收15-ketosterol，加以代謝，94%之代謝
  物排出細胞外。
  15-ketosterol之代謝可分三類：其一為水溶性代謝物，其二為脂溶極性伐謝物，其三為脂化合物。其百分率分布順
  次為61%，26%，及10%。細胞分泌物中含此三者代謝物，而前兩者占95%。
  本報告明示：肝腫瘤細胞（Hep G2）極具代謝及分泌之活性，可供對15-ketosterol代謝及控制分泌作進一步之研究。者
• 英文摘要： We demonstrated that two-thirds of [2,4-3H] 5α-cholest-8(14)-en-3β-ol-15-one was efficiently takenup and consequently metabolized and secreted by Hep G2 cells when it was pulsed for 16 hrs follwed bychasing for another 72 hrs. The metabolism was clearly reflected by the cellular secretion. Approximately61%, 26% and 10% of uptaken [2,4-3H] 5α-cholest-8(14)-en-3β-ol-15-one was metabolized to its water-soluble metabolites, polar metabolites in lipid phase and ketosteryl esters, respectively. Ninty-four percentof these metabolites was secreted into media. Interestingly, polar forms of the metabolites of 5α-cholest-8(14)-en-3β-ol-15-one accounted for over 95% of the cellular secretes. Limited secretion of ketosterylesters was also detected. The data strongly suggest that Hep G2 cells have the potential to process 5α-cholest-8(14)-en-3β-ol-15-one and could provide a good model for studying its secretion.
• 中文關鍵字： Hep G2 Cells; metabolism; bile secretion; oxysterol
• 英文關鍵字： --