• 作者： 應靜雯; 邵國銓; 馮濟敏
• 作者服務機構： 國立陽明醫學院生化研究所
• 中文摘要： 青蛙食道之抽取液中發現存在一胃蛋白?之抑制素。這抑制素與青蛙胃蛋白?原緊密結合，兩者都在65℃時急速失
  去活性，並且無法以色層分析法（phosphocellulose, DEAF-cellulose, hydroxylapatite及Sephadex G-75）加以分開。當
  青蛙胃蛋白?原純化後，這抑制素仍然連在一起。在pH2室溫培育10分鐘，青蛙胃蛋白?原活化成胃蛋白?之情況下，
  以三氯乙酸將反應液處理後，可在上清液得到相當數量之抑制物質。這抑制物質在65℃很穩定，但在95℃下2分鐘即
  失去百分之50之抑制能力。這些結果顯示出當初在青蛙食道組織抽取液中發現之抑制素，很可能就是青蛙胃蛋白?原之
  活化胜?。
• 英文摘要： An endogenous pepsin inhibitor was found to exist in the extract of frog esophagus. The inhibitorwas closely associated with the frog pepsinogen. Both were readily inactivated by heating at 65℃ and wereinseparable by chromatography on phosphocellulose, DEAE-cellulose, hydroxylapatite and Sephadex G-75.When frog pepsinogen was purified to homogeneity as judged by gel electrophoresis in the presence ofsodium dodecyl sulfate, the inhibitor remained associated. Whereas activation at pH 2 for 10 min at 25℃converted frog pepsinogen to pepsin, a considerable amount of inhibitory substance was produced in thetrichloroacetic acid-soluble fraction of the activation mixture. The inhibitory substance thus obtained wasstable at 65℃, but lost 50% of its inhibitory activity in 2 min at 95℃. These results seem to suggest thatthe inhibitor found in the extract of frog esophagus may be accounted for by an activation peptide or ac-tivation fragments of frog pepsinogen.
• 中文關鍵字： pepsin inhibitor; frog; activation peptide; pepsinogen
• 英文關鍵字： --