- 作者: Chu-Shek Lo
- 作者服務機構: Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, Md., USA
- 中文摘要: --
- 英文摘要: Regulation of (Na+ + K+)-adenosine triphosphatase (NaK-ATPase) by platelet-derived growth factor (PDGF) in cultured rat thoracic aortic smooth muscle cells (SMC) was examined. PDGF-BB enhances SMC proliferation and NaK-ATPase activity. Ouabain, an inhibitor of NaK-ATPase activity, prevents PDGF-BB-induced SMC proliferation. As shown by Western blot and immunochemiluminescence analysis, PDGF-BB also enhances α1, truncated α1, and β1 NaK-ATPase subunit levels. PDGF-AA and PDGF-AB show no effect on a1 and truncated α1 levels in slot blot analysis. Induction of NaK-ATPase subunit levels by PDGF-BB could be one of the initial events in vascular SMC proliferation.
- 中文關鍵字: --
- 英文關鍵字: Platelet-derived growth factors ; NaK-ATPase; Vascular smooth muscle cells; Ouabain; Proliferation