- 作者: Shwu-Bin Lina, Chia-Mau Wub, Wei-Chen Linc and Lou-sing Kanb,c
- 中文摘要: Triple stranded DNA helix (triplex) formation is limited by pH and the sequence of oligodeoxyribonucleotide. In this study, the 8-oxo-2?/font>-deoxyadenosine (8-oxoA) can replace 2?/font>-deoxycytidine for forming a base triad with a base pair guanine and cytosine. The target for the triplex formation is a motif of a promotor (5?/font>-d-TTGGGGGGGAA, -3 — -12 of PKCa m-RNA) of mouse protein kinase Ca. The probes contain 20 bases with a common sequence 5?/font>-d-XXXXXXTTCCCCCCAA. The XXXXXX portion may contain either 5-methyl-2?/font>-deoxycytidines, six 8-oxoA’s or both. Only the probe with a motif of six 8-oxoA’s can for triplex with the target in the presence of magnesium ion and spermine at neutral pH. Therefore, 8-oxoA is useful for triplex formation under physiological conditions.
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