- 作者: M. Iqbal Baba; Deepak Kaul; Anil Grover
- 作者服務機構: 1 Department of Experimental Medicine & Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India; ; 2 Department of Cardiology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
- 中文摘要: --
- 英文摘要: Since receptor/transcription factor family especially peroxisome proliferator-activated receptors PPARs (α,γ) and liver X receptor α (LXRα) have been recognized to play crucial role in both lipid metabolism and inflammation, the present study was addressed to explore the interrelationship between blood cellular genomic expression profile, serum lipid levels and severity of coronary heart disease (CHD) in human subjects. Based upon the demographic and laboratory data, the human subjects were divided into 4 groups. Genomic expression profile in the subjects belonging to these groups was determined by measuring the transcriptional expression of genes coding for PPARs (α, γ), CD36, LXRα and low density lipoprotein receptor (LDLR) in their blood mononuclear cells. This genomic expression profile was correlated with serum lipid profile as well as with the severity of CHD (revealed by coronary angiography coupled with modified Gensini score) using standard statistical analytical methods. Further in vitro and in vivo effect of statins on such genomic profile was also explored. Although genes coding for PPARs (α, γ), CD36, LDLR showed correlation with the severity of coronary atherosclerosis , blood cellular LXRα genomic profile showed conspicuous negative correlation with the severity of coronary atherosclerosis in subjects with or without hypercholesterolemia. This view was further confirmed in experiments directed to understand the effect of statins on the cellular genomic profile of PPARs (α, γ) and LXRα. Based on these reported findings, we propose that blood cellular LXRα genomic profile has a protective effect against the development of CHD and hence may be of importance in devising synthetic therapeutic drugs for CHD in future.
- 中文關鍵字: --
- 英文關鍵字: blood, coronary heart disease, cellular genomics, interrelationship, lipid