• 作者： Syu, Mei J.; Chen, Y.H.
• 中文摘要： 本研究工作是針對以Bacillus amyloliquefaciens之.alpha.型澱粉水解酵素發酵中不同碳源之效應進行探討。以批式發酵實驗進行,發酵液分別分析細胞密度,還原糖濃度,以及酵素活性。初始濃度為10g/l的葡萄糖,麥芽糖、木糖、澱粉分別作為限制基質進行討論。以葡萄糖為碳源可得較高菌體密度及較高之比生長速率。最大菌體密度為0.86g/l,而比生長速率為0.41h/sup -1/,然而以澱粉為碳源之培養卻可獲得較高之酵素活性與比酵素活性,分別為1089U /ml與1921(U/ml)(g/l)。同時,亦可獲得較大之比酵素生成速率。然而以葡萄糖為基質之培養,酵素活性之穩定度可維持地較好。經由對這些碳源培養下之發酵表現進行比較,澱粉因(1)酵素活性最高,(2)比酵素活性最高,(3)比酵素生成速率最高,(4).alpha.型澱粉水解酵素之生成可促使澱粉基質之水解以致於消耗利用,於是根據以上各點表現可結論為是諸碳源中對此發酵最佳之限制基質。
• 英文摘要： The aim of this work is to investigate the effect of different carbon sources on .alpha.-amylase fermentation with Bacillus amyloliquefaciens. Batch fermentations were carried out. The broths were analyzed for cell density, reducing sugar concentration, and enzyme activity. An initial concentration of around 10g/l of glucose, maltose, xylose, and starch were individually used as the respective substrates for studies. Higher cell density and higher specific growth rate were obtained from glucose. Maximum cell density of 0.86g/l can be reached from glucose with a specific growth rate of 0.41h /sup -1/. However, higher enzyme activity and higher specific enzyme activity were obtained from starch with respective maxima of 1089U/ml and 1921 (U/ml)/(g/l). Higher specific enzyme formation rate was also performed by starch substrate. Nevertheless, a better stability of enzyme activity was observed for glucose. Fermentation performance from different aspects by these substrates was thus compared. Starch was then determined, by the highest in (1) enzyme level produced, (2) specific enzyme level formed, (3) specific enzyme formation rate, and also by (4) the acceleration of hydrolysis and therefore the utilization of substrate, as the best carbon source among these substrates for this fermentation.
• 中文關鍵字： 澱粉; 發酵; 碳; 細胞密度; 糖
• 英文關鍵字： Amylase; Fermentation; Carbon; Cell Density; Sugar