• 作者： 林榮耀; 劉素瑩
• 作者服務機構： 國立臺灣大學醫學院生化學研究所
• 中文摘要： 由蓖麻子分離出之蓖麻子毒蛋白為一種醣結合蛋白，用親和性膠電泳分析法，可研究該蛋白與半乳醣及其誘導物之親和性。加含半乳醣之巨大分子之瓊脂於電泳膠中，觀察醣結合醣白質，在電泳中移動率減慢之情形。結果顯示蓖麻子毒蛋白中以蓖麻子毒蛋白－C因與瓊脂之親和力最大，而移動力最慢；蓖麻子毒蛋白－B親和力次之，而蓖麻子毒蛋白A之親和力最小，其電泳之移動率影響最小。如加半乳醣在電泳膠中，影響蓖麻子毒蛋白之移動率與瓊脂類似。 由其影響電泳移動率，可以計算出蓖麻子毒蛋白之Kd值；結果為蓖麻子毒蛋白C，B及A，各為18.32μM，28.13μM及52.88μM，而蓖麻子凝集素為4.32μM。親和性膠電泳為研究醣結合蛋白與醣類結合之情形，此方法簡單，可靠而快為其特徵。
• 英文摘要： The affinity gel electrophoresis of lectins purified from the seeds of Ricinus communis was studied.The mobilities of lectins on the gel showed various degree of retardation with their affinity toward theirmacromolecular ligand-agarose. Ricinus agglutinin which possessed the strongest affinity was retardedstrongly, ricin C, and ricin B moderatly, and ricin A, weakly. The concentrations of free moving ligand-galactose to reduce the retardation were also correlatedvery well with the potencies of affinities of lectins toward the ligand. The Kd values calculated from theretardations were 4.32 μM, 18.32 μM, 28.13 μM and 52.88 μM for ricinus agglutinin, ricin C, ricin B andricin A respectively. Affinity gel electrophoresis was found to be a simple and quick method for studyingthe interaction of lectins with their ligands.
• 中文關鍵字： affinity electrophoresis; isoricins and agglutinin; agaros; Kd
• 英文關鍵字： --