- 作者: 蕭明熙 ; 林麗娟
- 作者服務機構: 榮民總醫院醫學研究部
- 中文摘要: The chromatographic behavior of 8 lanostanoid triterpenes including 4 pairs of C-3 epimers and 2 pairs of C-3/C-15 positional isomers was studied by reversed phase HPLC. A consistent elution sequence of these paired isomers in all combination of three distinct solvent systems was observed. The mobile phase selectivity to separate specific, paired isomers was mainly dependent on the polarity difference contributed by C-3/C-15 hydroxyl and acetoxyl groups. The stereochemistry at C-3 played more profound role in guiding the hydrophobic interaction with C/sub 18/ stationary phase in the separation of C-3/C-15 positional isomers.
- 英文摘要: --
- 中文關鍵字: Separation; Positional Isomers; Hplc
- 英文關鍵字: 分離;位置異構物;高效液相層析法