- 作者: Lu-Ping Chow; Masaharu Kamo; Jung-Yaw Lin; Shiuan-Huei Wang; Yoshio Ueno; Akira Tsugita
- 作者服務機構: a Research Institute for Biosciences, Science University of Tokyo, Yamazaki, Noda, ; b Faculty of Pharmaceutical Sciences,Science University of Tokyo, Shinjuku,Tokyo, Japan; ; c Institute of Biochemistry, College of Medicine, National Taiwan University,Taipei, Taiwan
- 中文摘要: --
- 英文摘要: In this study, we sequenced a new type I ribosome-inactivating protein, trichoanguina, from the seeds of Trichosanthes anguina (snake gourd). Trichoanguina is a basic glycoprotein having an apparent molecular mass of 35.0 kD and possessing strong ribosome-inactivating activity. Trichoanguina was cleaved with cyanogen bromide and partially digested with thermolysin, chy-motrypsin, trypsin and Staphylococcus aureus V8 protease. The subsequent peptide fragments were separated by SDS-polyacrylamide gel electrophoresis, followed by electroblotting to polyvinylidene difluoride membranes and then sequencing. The sequencing of trichoanguina was completed, consisting of 245 amino acid residues. The sequencing of trichoanguina revealed a considerable homology to trichosanthin and a-trichosanthin, which are known as abortifacient, ribosome-inactivating and antihuman immunodeficiency virus proteins, with 46.7% and 55.6% amino acid identities, respectively. The sequence conserves two active sites: Glu-158 and Arg-161.
- 中文關鍵字: --
- 英文關鍵字: Ribosome-inactivating protein(s) ; Trichosanthes anguina ; Trichoanguina ; Protein sequence