- 作者: --
- 作者服務機構: Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
- 中文摘要: A highly sensitive and specific fluorimetric method was developed for the determination of ethionamide in pharmaceutical preparations and biological fluids (urine and plasma). The proposed method is based on the study of the quantitative quenching effect of ethionamide on the native fluorescence of eosin in acidic medium due to complex formation. The fluorescence intensity was measured at 536 nm (after excitation at 337 nm). The different experimental parameters affecting the quenching of the native fluorescence of eosin were carefully studied and incorporated into the procedure. Under the described conditions, the method is applicable over the concentration range of 1-8 mg/mL with a detection limit of 80 ng/mL. The percentage recoveries for dosage form, spiked urine and plasma were 99.38 ± 0.824, 99.44 ± 1.58 and 99.25 ± 1.15%, respectively. The results obtained were in good agreement with the official methods. A proposal of the reaction pathway is suggested.
- 英文摘要: --
- 中文關鍵字: Ethionamide; Eosin; Fluorometery; Quenching; Biological fluids.
- 英文關鍵字: --