- 作者: Alireza Tahamtan, Amir Ghaemi, Ali Gorji, Hamid R Kalhor, Azadeh Sajadian, Alijan Tabarraei, Abdolvahab Moradi, Fatemeh Atyabi, Mishar Kelishadi
- 作者服務機構: Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
- 中文摘要: --
- 英文摘要:
Background:
Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability.
Results:
The transfection of CS-pEGFP NPs was efficient in CHO cells and the expression of green fluorescent proteins was well observed. In addition, NCS-DNA E7 vaccine induced the strongest E7-specific CD8+ T cell and interferon γ responses in C57BL/6 mice. Mice vaccinated with NCS-DNA E7 vaccine were able to generate potent protective and therapeutic antitumor effects against challenge with E7-expressing tumor cell line, TC-1.
Conclusions:
The strong therapeutic effect induced by the Chitosan-based nanodelivery suggest that nanoparticles may be an efficient carrier to improve the immunogenicity of DNA vaccination upon intramuscular administration and the platform could be further exploited as a potential cancer vaccine candidate in humans. - 中文關鍵字: --
- 英文關鍵字: Human papilloma virus; E7; DNA vaccine; Chitosan; Tumor