• 作者： Liu, Der-Zen; Chen, Wen-Yih; Tsai, Li-Min; Yang, Shu-Ping
• 中文摘要： 本研究利用雷射粒徑儀以及酵素染色探討膽固醇之嵌入卵磷脂微脂粒脂雙層系統中對其粒徑變化及游離磷脂質之影響與機制之探討,並調整溫度值以了解溫度對微脂粒之物理穩定性的影響。實驗結果證實膽固醇之嵌入將改變微脂粒脂雙層之表面性質, 在界面電位上, 膽固醇之嵌入將提昇了微脂粒間之斥力場作用; 關於脂質分子間之影響, 膽固醇之嵌入有助於增加脂雙層內之脂質分子排列穩定度, 此外, 由於膽固醇之頭基與脂質分子間有氫鍵之形成更將有助於提昇脂質分子間之引力進而降低磷脂質之游離釋放以提昇脂雙層之穩定性。溫度方面, 溫度提高除了降低界面電位(降低微脂粒間之斥力場),亦會降低脂雙層內脂質分子之排列穩定度。
• 英文摘要： This study demonstrates that the addition of cholesterol enhances the physical stability of liposome illustrated via the structure of the bilayer and the interaction force between the liposomes. Experimental results indicate that the addition of cholesterol increases the absolute zeta-potential and the electrostatic repulsive interaction or forces between phosphatidylcholine (PC) liposomes. From a structural perspective, the incorporation of cholesterol can increase the rigidity of the bilayer. Additionally, the concentration of free PC lipids was decreased when the cholesterol was incorporated into the PC liposomal bilayer, indicating that the addition of cholesterol can enhance the intravesicle attractive interaction. Moreover, regarding the impact of shear force on liposomal stability, we found that higher shear force applied to the liposome suspension increases the concentration of free lipids. However, the impact of shear force on liposomal stability is minimized with the addition of cholesterol.
• 中文關鍵字： 微脂粒; 膽固醇; 界達電位; 游離磷脂質; 剪力
• 英文關鍵字： Liposome; Cholesterol; Zeta-Potential; Free-Lipid; Shear Force