- 作者: Jobin Mathew, Savitha Balakrishnan, Sherin Antony, Pretty MARY Abraham and C S Paulose
- 作者服務機構: Department of Zoology, CMS College Kottayam, Kerala, India
- 中文摘要: --
- 英文摘要: Background Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue. Methods In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated. Results Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P<0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAalpha1, GABAAgamma, GABAAdelta, GABAB and GAD where down regulated (P<0.001) in epileptic rats. GABAAalpha5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance. Conclusions Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.
- 中文關鍵字: --
- 英文關鍵字: Epilepsy, Bacopa monnieri, Bacoside-A, Pilocarpine, Carbamazepine