• 作者： Chang-Yen; Chen, Chiun-Nan; Sung, Hsing-Wen; Chiu, Yung-Tsung; Lu, Jen-Her
• 中文摘要： 在本研究裡，我們使用了環氧化物交聯處理的豬心包膜來做為修補狗肺動脈的人工補綴片。為了抑制此人工補綴片內側與血液接觸後的可能產生血栓，以及補綴片外側與肺臟接觸後的可能產生組織的沾黏現象，我們分別使用了兩種不同鍵結的方式(離子鍵結和終端共價鍵結)將肝素接枝在人工補綴片上。實驗中的對照組，是同樣以環氧化物交聯處理但不做肝素接枝的人工補綴片。實驗的結果顯示，人工補綴片在接枝肝素後，能減少血栓的產生以及組織沾黏的現象，而以終端共價鍵結肝素的方式，由於其鍵結比較穩定，效果比較顯著。另外，我們也發現經肝素接枝後的人工補綴片，較未經肝素接枝的補綴片能抵抗體內分解，但是肝素接枝並無法改善植入組織鈣化的現象。
• 英文摘要： Heterologous pericardia, acquired from mammal, are often used as biological patches in cardiovascular surgery for pericardial substitute, intracardiac defect repair, stenosis enlargement, and change of blood stream direction. However, these biological patches have been clinically encountered with thrombus formation and tissue adhesion. These concerns may be overcome by immobilizing heparin on the surfaces of these biological patches. The study was to evaluate an epoxy-fixed porcine pericardium with ionically- or covalently-immobilized heparin implanted in a canine model as a vascular patch. The nonheparinized counterpart was used as a control. The results obtained suggested that surface-immobilized heparin may reduce thrombus formation and tissue adhesion of implanted biological patches. Additionally, the porcine pericardium with covalently-immobilized heparin was relatively better than its ionically-immobilized counterpart in reducing thrombus formation and tissue adhesion. Therefore, it is suggested that heparin be immobilized by stable covalent bond in clinical use. The resistance against in vivo degradation for the heparinized tissues (ionically- or covalently- immobilized) was relatively better than the nonheparinized tissue. However, heparinization did not seem to prevent the implanted biological tissues from calcification.
• 中文關鍵字： 生物貼劑; 離子性固定化肝素; 共價性固定性肝素; 血栓; 組織黏膠
• 英文關鍵字： Biological Patch; Ionically Immobilized Heparin; Covalently Immobilized Heparin; Thrombus; Tissue Adhesive