- 作者: Tzong-Yueh Chen Chia Po Lin Chien-Chang Loa Tso-Ling Chen Huey-Fang Shang Jaulang Hwang Cho-Fat Hui
- 作者服務機構: Institute of Genetics, School of Life Science, National Yang-Ming University, INstitute of Molecular Biology, Academia Sinica, Divison of Drug biology, National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, graduate School of Microbiology, Soochow University, Department of Medicine, Taipei Medical College, and Institute of Zoology, academia Sinica, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Pseudomonas exotoxin A(PE)is one of the most potentcytotoxic agents produced by Pseudomonas aeruginosa.In this study, we examined the possibility of using PEwith a deletion of 38 carboxyl-terminal amino acid resi-dues, designated PE(Δ576-613), for active immunizationagainst PE-mediated disease. We first examined thetoxic effects of PE and PE (Δ576-613) on 5- and 9-week-Old ICR mice. The results show that the subcutaneousadministration of PE(Δ576-613) at a dose of 250μg wasstill nontoxic to 5- and 9-week-old ICR mice, while nativePE was lethal at a dose of 0.5 and 1 μg, respectively.PE(Δ576-613) was then used to immunize ICR mice. Theminimum dose of PE(Δ576-613)that could effectivelyinduce anti-PE antibodies in 5- and 9-week-old ICR micewas found to be 250 ng. However, immunization with250 ng PE(Δ576-613) failed to protect the immunizedmice from a lethal dose of PE. The effective immuniza-tion dose of PE(Δ576-613) that could protect miceagainst a 2 μg PE challenge was found to be 15 μg.Inaddition, sera obtained from PE(Δ576-613) -immunizedICR mice were able to neutralize PE intoxication andeffectively protect mice from PE. Thus, PE(Δ576-613)may be used as an alternative route to new PE vaccinedevelopment.
- 中文關鍵字: --
- 英文關鍵字: Pseudomonas exotoxin A. Vaccination