- 作者: Sai-Wen Tang; Wen-Hsin Chang; Yu-Wei Chao; Chung-Yei Lin; Hsiao-Fen Chen; Yen-Han Lai; Bo-Wen Zhan; Yih-Ching Su; Sen-Wen Jane; Yu-Chi Chen; Chyong-Ing Hsu; Wei-Chou Lin; Kao-Chung Wang; Ming-Kuen Lai; Jung-Yaw Lin
- 作者服務機構: 1 Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, R.O.C; ; 2 Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C; ; 3 Department of Urology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要: Renal cell carcinoma (RCC) is the most common malignancy in adult kidney, and accounts for 3% of malignancies worldwide with increasing incidence. Clear cell RCC (ccRCC) is the major type in RCC Resection by surgery is the main treatment because the response of ccRCC to traditional therapies is very poor. To identify the tumor-associated genes for better understanding the molecular mechanism of ccRCC, the full-length enriched cDNA libraries of ccRCC and normal kidney tissues were constructed by the oligo-capping method. Nucleotide sequences of the cDNA libraries of ccRCC and normal kidney tissues were sequenced. From the sequence analysis of 19,425 and 12,400 clones of ccRCC and normal kidney tissues, 4356 and 3055 genes were identified, respectively. By comparing the gene-expression patterns of ccRCC and normal tissues, the up- or down-regulated genes were identified. Among these identified genes, the differential expression of annexin A2 and argininosuccinate synthetase genes were further confirmed by quantitative real-time PCR and Western blot analysis.
- 中文關鍵字: --
- 英文關鍵字: clear cell renal cell carcinoma, full-length cDNA library, Annexin A2, argininosuccinate synthetase