- 作者: Thoppil R Anju; Sadanandan Jayanarayanan; Cheramadatikudiyil S Paulose
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kerala, India
- 中文摘要: --
- 英文摘要:
Background- Hypoxia during the first week of life can induce neuronal
death in vulnerable brain regions usually associated with an impairment of
cognitive function that can be detected later in life. The neurobiological changes
mediated through neurotransmitters and other signaling molecules associated with
neonatal hypoxia are an important aspect in establishing a proper neonatal care.
Methods- The present study evaluated total GABA, GABAB receptor
alterations, gene expression changes in GABAB receptor and glutamate
decarboxylase in the cerebellum and brain stem of hypoxic neonatal rats and the
resuscitation groups with glucose, oxygen and epinephrine. Radiolabelled GABA
and baclofen were used for receptor studies of GABA and GABAB receptors
respectively and Real Time PCR analysis using specific probes for GABAB
receptor and GAD mRNA was done for gene expression studies.
Results- The adaptive response of the body to hypoxic stress resulted in a
reduction in total GABA and GABAB receptors along with decreased GABAB
receptor and GAD gene expression in the cerebellum and brain stem. Hypoxic
rats supplemented with glucose alone and with oxygen showed a reversal of the
receptor alterations and changes in GAD. Resuscitation with oxygen alone and
epinephrine was less effective in reversing the receptor alterations.
Conclusions- Being a source of immediate energy, glucose can reduce the
ATP-depletion-induced changes in GABA and oxygenation, which helps in encountering hypoxia. The present study suggests that reduction in the GABAB
receptors functional regulation during hypoxia plays an important role in central
nervous system damage. Resuscitation with glucose alone and glucose and
oxygen to hypoxic neonatal rats helps in protecting the brain from severe hypoxic
damage. - 中文關鍵字: --
- 英文關鍵字: GABAB; neonatal hypoxia; cerebellum and brain stem