- 作者: Sung Eun Kim; Oju Jeon; Jung Bok Lee; Min Soo Bae; Heoung-Jae Chun; Seong-Hwan Moon; Il Keun Kwon
- 作者服務機構: Department of Oral Biology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, South Korea
- 中文摘要: --
- 英文摘要:
This study was performed to determine if a combination of previously undifferentiated bone marrowderived mesenchymal stem cells (BMMSCs) and exogenous bone morphogenetic protein-2 (BMP-2) delivered via heparin-conjugated PLGA nanoparticles (HCPNs) would extensively regenerate bone in vivo. In vitro testing found that the HCPNs were able to release BMP-2 over a 2-week period. Human BMMSCs cultured in medium containing BMP-2-loaded HCPNs for 2 weeks differentiated toward osteogenic cells expressing alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) mRNA, while cells without BMP-2 expressed only ALP. In vivo testing
found that undifferentiated BMMSCs with BMP-2-loaded HCPNs induce far more extensive bone formation than either implantation of BMP-2-loaded HCPNs or osteogenically differentiated BMMSCs. This study demonstrates the feasibility of extensive in vivo bone regeneration by transplantation of undifferentiated BMMSCs and BMP-2 delivery via HCPNs. - 中文關鍵字: --
- 英文關鍵字: Bone marrow-derived mesenchymal stem cell; Bone morphogenetic protein-2; Bone regeneration; Heparin-conjugated PLGA nanoparticles