- 作者: 程定璽,王韶華,劉文傑
- 作者服務機構: Key Laboratory of Environmental Science and Engineering Education Commission of Henan, Department of Chemistry, Henan Normal University, Xinxiang, Henan 453007, P. R. China
- 中文摘要: 4,5-Dibromofluorescein(R) binding to proteins causes a decrease in the fluorescence maximum of R at 530 nm; the intensity of fluorescence quenching is directly proportional to protein content, Based on this, a new fast and simple fluorescence quenching method for the determination of proteins was developed. Under experimental conditions, the linear range of this assay is 0~15 mg/L and the detection limit is 40 μg/L. The method has been applied to the analysis of human serum samples and gave values close to those obtained by the Coomassie brilliant blue G-250 (CBB G-250) method, which indicated that the method is not only high in sensitivity but also reliable.
- 英文摘要: --
- 中文關鍵字: 4,5-Dibromofluorescein; Proteins; Fluorescence quenching; Fluorescence and Absorption spectra.
- 英文關鍵字: --