- 作者: Tzong-Hsien Lee; Dar-In Tai; Cha-Ju Cheng; Chi-Shu Sun; Ching-Yih Lin; Ming-Jen Sheu; Wei-Ping Lee; Cheng-Yuan Peng; Andrew H-J Wang; Sun-Lung Tsai
- 作者服務機構: 1 Liver Research Unit, Chang-Gung Memorial Hospital, Linko, Taiwan; ; 2 Hepatogastroenterology Section, Department of Internal Medicine, Chi Mei Medical Center, 901 Chung-Hwa Rd, Yung Kang City, Tainan, 710, Taiwan; ; 3 Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; ; 4 Hepatogastroenterology Section, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan; ; 5 Core Facilities for Proteomics Research and Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan; ; 6 Department of Biochemistry and Molecular Biology, Monash University, P.O. Box 13D, Clayton Vic, 3800, Australia
- 中文摘要: --
- 英文摘要: Chronic infections with hepatitis B and C viruses (HBV and HCV) are etiologically linked to hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). Both viruses may induce activation of nuclear factor-kappa B (NF-κB) in hepatocytes that plays a crucial role in the regulation of cell growth and apoptosis. Functional proteomics analysis of proteins associated with NF-κB signaling complexes in both viruses-related HCC tumor and non-tumor tissues may disclose possible common mechanisms in hepatocarcinogenesis. By functional proteomics, we analyzed proteins associated with NF-κB-signaling complexes in four-paired human HCC tumor and non-tumor tissues from HBV- and HCV-infected patients, respectively, and in one-paired tissue with dual viral infection. The quantity of NF-κB-associated proteins was semi-quantitatively measured by protein spot intensity on the gels of two-dimensional polyacrylamide gel electrophoresis. The results showed that overexpression of NF-κB-associated Wnt-1 protein in tumor part was detected in the majority of HBV- and HCV-infected HCC samples. These data suggest that enhanced expression of NF-κB-associated Wnt-1 protein might be a mechanism of hepatocarcinogenesis common to HBV- and HCV-infected patients. NF-κB signaling pathway and Wnt-1 protein could be potential targets for designing highly effective therapeutic agents in treating HCC and for chemoprevention of hepatocarcinogenesis.
- 中文關鍵字: --
- 英文關鍵字: functional proteomics, hepatitis B virus, hepatitis C virus, hepatocarcinogenesis, hepatocellular carcinoma, nuclear factor-κB, Wnt-1 protein