- 作者： 沈明琛
- 作者服務機構： 康乃爾大學醫學院生化系及紐約血液中心核酸實驗室
- 中文摘要：
eIF-3早已為人所知有分離之功能，主要是將80S核糖體分成40S及60S核糖單體。同時eIF-3與40S有甚強之親
和力亦已證明。唯eIf-3與60S核糖單體有否親和力尚欠文獻。本篇即提供此親和力之證據。eIF-3是由兔子網織血球
中取得，將其接在 Sepharose 上即可作層析之用。用大白鼠之肝核糖體或兔子網織血球之核糖體加在eIF-3-Sepharose
上作親和層析，60S核糖單體均可與之結合。而40S核糖單體與其之結合能力更強。60S核糖單體可被0.5 M KCl完全
析出而40S核糖單體?須 1.0 M KCl才能完全被析出。由此可見，有二種結合，其一為微力結合，另一為強力結合。但
是，未經0.5 M KCl處理之核糖體?甚少強力結合之情形。
通訊處：台北市郵政八二四四號信箱
國防醫學院生物化學系l - 英文摘要： It has already been established that eIF-3 has an ability to dissociate 80S ribosomes into 40S and60S ribosomal subunits and subsequently bind to 40S. However eIF-3 and 60S interactions have neverbeen demonstrated. In this study, Rabbit reticulocytes eIF-3 were coupled with Sepharose 4B for the purpose of creatingan affinity chromatographic column, and subsequently studying the interaction between eIF-3 and ribo-somes. It was then demonstrated that 60S ribosomal subunits binds to eIF-3. In comparing the 40S and60S ribosomes, this column preferentially binds 40S ribosomal subunits. The 40S ribosomal subunits werenot completely eluted until the KCl concentration was raised to 1.0 M. On the other hand, the 60S ribo-somal subunits could be easily eluted with 0.5 M KCl. When ribosomes that had never been exposed to0.5 M KCl (i.e., native ribosomes) were applied to the column, very little 40S ribosomal subunits were elut-ed with 1.0 M KCl. Furthermore, polysome derived ribosomes had similar properties to native ribosomes.These results suggest that 0.5 M KCl-washed ribosomes were different from native as well as from poly-some-derived ribosomes.
- 中文關鍵字： --
- 英文關鍵字： --
