• 作者： 陳昇明; 曾振南
• 作者服務機構： 國立中興大學植物學研究所
• 中文摘要： 由本省土壤中分離一株蠟樣桿菌（Bacillus cereus CHU 130），培養於黃豆粉甘露醇培養基可產生一種抗真菌抗生物質，定名為『蠟樣桿菌素』（bacereutin）。蠟樣桿菌素可經由吸附管柱（Amberlite XAD-2）、陽離子交換樹脂管柱（Dowex50W-X8）和膠體過濾管柱（Sephadex LH20和Biogel P-2）純化分離。蠟樣桿菌素為水溶性，微溶於甲醇和87%丙酮，不溶於正丙醇、正丁醇、丙酮、苯、醋酸乙酯等溶劑。蠟樣桿菌素相當穩定，經高溫處埋或處於各種酸鹼值，仍然保持抗生活性。它在薄層色層分析圖上與茆三酮（ninhydrin）具呈色反應。 蠟樣桿菌素可抑制酵母菌（Saccharomyces cerevisiae CHU 1）、擬青黴菌（Paecilomyces variotii CHU 6）、米黑毛黴菌（Rhizomucor miehei　CHU 40）和鐮孢菌（Fusarium oxysporum CHU 98）等真菌生長。然而，在抑制真菌有效濃度下，抗生物質對於枯草桿菌（Bacillus subtilis CHU 93）和大腸菌（Escherichia coli CHU 96）並無抑制效應。蠟樣桿菌素是一種茆三酮陽性之抗真菌抗生素。
• 英文摘要： An antifungal metabolite, bacereutin, was isolated from culture filtrate of Bacillus cereus CHU 130.The bacterium was isolated from soils collected in Changhwa County, Taiwan, and was grown in soybeanmeal-mannitol broth for production of the antibiotic metabolite. The antibiotic metabolite was isolatedby adsorption column chromatography of Amberite XAD-2 and was purified by passing through thechromatographic columns packed with Dowex 50W-X8, Sephadex LH 20 and Biogel P-2. The antibiotic metabolite was soluble in water and 87% acetone, and was slightly soluble inmethanol, but was not dissolved in n-propanol, n-butanol, acetone, benzene and ethyl acetate. The anti-biotic metabolite was a heat-stable and ninhydrin-positive substance. The antibiotic activities of bacereutin were tested by means of the agar-diffusion plate method. Theantibiotic metabolite inhibited the growth of Saccharomyces cerevisiae CHU 1, Paecilomyces variotiiCHU 6, Rhizomucor miehei CHU 40 and Fusarium oxysporum CHU 98. Bacereutin was a ninhydrin-positive antifungal antibiotic.
• 中文關鍵字： bacereutin; antifungal antibiocic; Bacillus cereus
• 英文關鍵字： --