- 作者: Huey-Chuan Shih; Chun-Sen Hsu; Ling-Ling Yang
- 作者服務機構: School of Pharmacy, College of Pharmacy, Taipei Medical University, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Scutellariae Radix is one of the well-known tocolytic Chinese herbs. Oroxylin A is isolated from the
root of Scutellaria baicalensis. The main syndrome of preterm birth is caused by uterus contractions
from excitatory factors. Administration of tocolytic agents is a strategy to prevent the occurrence of
preterm births. The aim of this study was to investigate the effects of oroxylin A on contractions of
uterine strips isolated from non-pregnant female Wistar rats (250~350 g). Contractions of the uterus
were induced with acetylcholine (Ach) (1 μM), PGF2α (0.1 μM), oxytocin (10-3 U/ml), KCl (56.3
mM), tetraethylammonium (TEA; 1 and 10 mM), 4-aminopyridine (4-AP; 5 mM), glipizide (30
μM), a nitric oxide synthase (NOS) inhibitor (LNNA; 10-3 M), a β-receptor blocker (propranolol; 10
μM), and a cyclooxygenase inhibitor (indomethacin; 60 μM). The inhibitory effects of the amplitude
and frequency of spontaneous contractions by oroxylin A were antagonized with Ach (IC50 22.85
μM), PGF2α (IC50 27.28 μM), oxytocin (IC50 12.34 μM), TEA; 1 and 10 mM (IC50 52.73 and 76.43
μM), 4-AP (IC50 67.16 μM), and glipizide (IC50 27.53 μM), but oroxylin A was not influenced by
Ca2+-free medium, LNNA, propranolol, or indomethacin. Otherwise, oroxylin A-mediated
relaxation of the rat uterus might occur through opening of uterine calcium-dependent potassium
channels or adenosine triphosphate potassium channel activation. This suggests that oroxylin A is
the tocolytic principle constituent of Scutellariae Radix, and oroxylin A may provide a lead
compound for new tocolytic drug development in the future. - 中文關鍵字: --
- 英文關鍵字: --