• 作者： 翟建富
• 作者服務機構： 國立陽明醫學院生物化學研究所
• 中文摘要： 利用次選殖（Subcloning）和轉位子變異法（Transponson Mutagenesis），我們已將ColE3操縱子定位，並分析了cea，cei和cel基因之特性。將含有CoIE3操縱子的片段的重組質體轉入Maxicell中，我能在SDS-PAGE中分別觀察到一個60 kDa，14-15 kDa，9-10 kDa和4-5 kDa的ColE3，ImmE8，ImmE3和LysE3的蛋白。實驗結果證明，Maxicell系統是一個容易而簡單的方法來鑑定質體中所產生的蛋白。
• 英文摘要： The cea, cei, and cel genes of the ColE3 operon were localized and characterized by subcloning andby transposon mutagenesis. Plasmids containing different portions of the ColE3 operon were transformedinto an E. coli maxicell strain CSR603. Proteins encoded by these plasmids were analyzed by SDS-PAGE afterUV irradiation of the cells. The results showed that the molecular weights of Col, ImmE8, ImmE3 and Lyswere 60 kDa, 15 kDa, 10 kDa, and 4-5 kDa, respectively. These results indicated that the maxicell systemprovides an easy and simple method for the identification of plasmid-encoded proteins.
• 中文關鍵字： ColE3 Operon; cea gene; cei gene; cel gene; Expression; Maxicell; in-vivo gene expression.
• 英文關鍵字： --