- 作者: Zhang W; Tsurushima H; Oyane A; Yazaki Y; Sogo Y; Ito A; Matsumura A
- 作者服務機構: Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan
- 中文摘要: --
- 英文摘要:
Background: Safe and efficient gene transfer systems are needed for tissue engineering.
We have developed an apatite composite layer including the bone morphogenetic protein-2
(BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation.
Methods: An apatite composite layer was evaluated to determine the efficiency of gene
transfer to cells cultured on it. Cells were cultured on a composite layer including the
BMP-2 gene and FB, and BMP-2 gene expression, BMP-2 protein concentrations, alkaline
phosphatase (ALP) activity, and osteocalcin (OC) concentrations were measured. A bone
defect on the cranium of rats was treated with hydroxyapatite (HAP)-coated ceramic
buttons with the apatite composite layer including the BMP-2 gene and FB
(HAP-BMP-FB). The tissue concentration of BMP-2, bone formation, and the expression
levels of the BMP-2, ALP, and OC genes were all quantified.
Results: The apatite composite layer provided more efficient gene transfer for the cultured
cells than an apatite composite layer without FB. The BMP-2 concentration was
approximately 100~600 pg/mL in the cell-culture medium. Culturing the cells on the
apatite composite layer for 27 days increased ALP activity and OC concentrations. In
animal experiments, the tissue concentrations of BMP-2 were over 100 pg/mg in the
HAP-BMP-FB group and approximately 50 pg/mg in the control groups. Eight weeks
later, bone formation was more enhanced in the HAP-BMP-FB group than in the control
groups. In the tissues surrounding the HAP button, the gene expression levels of ALP and
OC increased.
Conclusion: The BMP-2 gene-FB-apatite composite layer might be useful for bone
engineering.
- 中文關鍵字: --
- 英文關鍵字: bone engineering, BMP-2 gene-fibronectin-apatite composite layer, BMP-2 gene therapy, non-viral gene transfer.