- 作者: Shui-Mei Wang; Yu-Fen Chang; Yi-Ming Arthur Chen; Chin-Tien Wang
- 作者服務機構: Institute of Public Health, National Yang-Ming University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: We replaced the HIV-1 nucleocapsid (NC) domain with different N-coding sequences to test SARSCoV nucleocapsid (N) self-interaction capacity, and determined the capabilities of each chimera to direct viruslike particle (VLP) assembly. Analysis results indicate that the replacement of NC with the carboxyl-terminal half of the SARS-CoV N resulted in the production of wild type (wt)-level virus-like particles (VLPs) with the density of a wt HIV-1 particle. When co-expressed with SARS-CoV N, chimeras containing the N carboxyl-terminal half sequence efficiently packaged N. However, the same was not true for the chimera bearing the N amino-terminal half sequence, despite its production of substantial amounts of VLPs. According to further analysis, HIV-1 NC replacement with N residues 2–213, 215–421, or 234–421 resulted in efficient VLP production at levels comparable to that of wt HIV-1, but replacement with residues 215–359, 302–421, 2–168, or 2–86 failed to restore VLP production to wildtype levels. The results suggest that the domain conferring the ability to direct VLP assembly and release in SARSCoV N is largely contained between residues 168 and 421.
- 中文關鍵字: --
- 英文關鍵字: SARS-CoV N; HIV-1 NC; Virus-like particle assembly