- 作者: Rene Chun; Hung Fan
- 作者服務機構: Department of Molecular Biology and; Biochemistry, and Cancer Research; Institute, University of California, Irvine, Calif., USA
- 中文摘要: --
- 英文摘要: Two independent pathways for gag gene expression exist in Moloney murine leukemia virus (M-MuLV). One begins with Pr65gag that is processed and cleaved into the internal structural proteins of the virion. The other pathway begins with the glycosylated gag polyprotein, gPr80gag. gPr80gag consists of Pr65gag plus additional N-terminal residues and it is glycosylated. A glycosy- lated-gag-negative mutant of M-MuLV (Ab-X-MLV) was previously con- structed and shown to replicate in tissue culture. To test for the importance of glycosylated gag in vivo, the Ab-X-MLV mutant was inoculated intraperitone- ally into newborn NIH Swiss mice. Mutant-infected mice developed typical lymphoblastic lymphomas at rates comparable to wild-type M-MuLV at either high (2 x 10^4 XC pfu/animal) or low (2 x 10^2 XC pfu/animal) doses. How- ever, when viral protein expression was examined in the resultant tumors, six out of six mice showed evidence of virus that had recovered gPr80gag expres- sion. These results suggest that glycosylated gag is important for M-MuLV propagation or leukemogenesis in vivo.
- 中文關鍵字: --
- 英文關鍵字: Moloney murine leukemia virus; Glycosylation; gag; Glycosylated gag; Retroviruses; RNA viruses